Autoperfused mouse flow chamber reveals synergistic neutrophil accumulation through P-selectin and E-selectin.

نویسندگان

  • Michael L Smith
  • Markus Sperandio
  • Elena V Galkina
  • Klaus Ley
چکیده

To study rolling of mouse neutrophils on P- and E-selectins in whole blood and without cell isolation, we constructed an autoperfused flow chamber made from rectangular microslides (0.2x2 mm) perfused from a carotid artery catheter. A differential pressure transducer served to measure wall shear stress. Green fluorescent neutrophils rolled on P-selectin but not E-selectin coated at 50 ng/ml, with some rolling on E-selectin at 150 ng/ml. However, when P- and E-selectins were coimmobilized, the resulting number of rolling neutrophils was sixfold and fourfold higher than on P- or E-selectin alone. Velocity and flux analysis shows that P-selectin initiates neutrophil rolling, and a small amount of E-selectin, unable to capture many neutrophils, reduces the rolling velocity of all neutrophils by more than 90%. The unexpected synergism between E- and P-selectins explains why neutrophil recruitment is enhanced when both selectins are expressed.

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عنوان ژورنال:
  • Journal of leukocyte biology

دوره 76 5  شماره 

صفحات  -

تاریخ انتشار 2004